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genepulser ii electroporation device (bio- rad, feldkirchen, germany)  (Bio-Rad)


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    Bio-Rad genepulser ii electroporation device (bio- rad, feldkirchen, germany)
    Genepulser Ii Electroporation Device (Bio Rad, Feldkirchen, Germany), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+genepulser+ii+electroporator/gene+pulser/10__7554_slash_elife__86100-332-15-19
    Average 90 stars, based on 1 article reviews
    genepulser ii electroporation device (bio- rad, feldkirchen, germany) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Small-Subunit rRNA Processome Proteins Are Translationally Regulated during Differentiation of Trypanosoma cruzi
    Article Snippet: .. Cells were transfected by electroporation in 4-mm cuvettes, using a Bio-Rad GenePulser II electroporator, with two pulses of 1,600 V and 25 μF. ..

    Article Title: Epstein-Barr Virus Represses the FoxO1 Transcription Factor through Latent Membrane Protein 1 and Latent Membrane Protein 2A
    Article Snippet: .. For transient transfections, 1 × 10 7 DG75 cells from a suspension culture were transfected by electroporation using a Bio-Rad Genepulser II electroporator at 300 V and 950 μF at room temperature in 500 μl growth medium. .. Following electroporation, the cells were transferred to 3.5 ml of fresh medium per sample and incubated at 37°C in a 5% CO 2 humidified incubator for 20 h. Total protein lysates were generated using the passive lysis buffer provided with the Promega Dual Luciferase reporter assay system (E-1910).

    Article Title: Functional Genomic Characterization of mRNAs Associated with TcPUF6, a Pumilio-like Protein from Trypanosoma cruzi
    Article Snippet: .. T. cruzi epimastigotes (5 107) were transfected with 50 g of vector DNA by electroporation in 4-mm cuvettes, using the Bio-Rad GenePulser II electroporator, with two pulses of 450 V and 500 millifarads. ..

    Article Title: Small-Subunit rRNA Processome Proteins Are Translationally Regulated during Differentiation of Trypanosoma cruzi
    Article Snippet: .. Cells were transfected by electroporation in 4-mm cuvettes, using a Bio-Rad GenePulser II electroporator, with two pulses of 1,600 V and 25 F. After 24 h, 5 g/ml phleomycin was added for the selection of transfected parasites. ..

    Article Title: Epstein-Barr Virus Represses the FoxO1 Transcription Factor through Latent Membrane Protein 1 and Latent Membrane Protein 2A
    Article Snippet: .. For transient transfections, 1 107 DG75 cells from a suspension culture were transfected by electroporation using a Bio-Rad Genepulser II electroporator at 300 V and 950 F at room temperature in 500 l growth medium. .. Following electroporation, the cells were transferred to 3.5 ml of fresh medium per sample and incubated at 37°C in a 5% CO2 humidified incubator for 20 h. Total protein lysates were generated using the passive lysis buffer provided with the Promega Dual Luciferase reporter assay system (E-1910).

    Article Title: Functional Genomic Characterization of mRNAs Associated with TcPUF6, a Pumilio-like Protein from Trypanosoma cruzi
    Article Snippet: .. T. cruzi epimastigotes (5 × 10 7 ) were transfected with 50 μg of vector DNA by electroporation in 4-mm cuvettes, using the Bio-Rad GenePulser II electroporator, with two pulses of 450 V and 500 millifarads. ..

    Electroporation:

    Article Title: Small-Subunit rRNA Processome Proteins Are Translationally Regulated during Differentiation of Trypanosoma cruzi
    Article Snippet: .. Cells were transfected by electroporation in 4-mm cuvettes, using a Bio-Rad GenePulser II electroporator, with two pulses of 1,600 V and 25 μF. ..

    Article Title: Epstein-Barr Virus Represses the FoxO1 Transcription Factor through Latent Membrane Protein 1 and Latent Membrane Protein 2A
    Article Snippet: .. For transient transfections, 1 × 10 7 DG75 cells from a suspension culture were transfected by electroporation using a Bio-Rad Genepulser II electroporator at 300 V and 950 μF at room temperature in 500 μl growth medium. .. Following electroporation, the cells were transferred to 3.5 ml of fresh medium per sample and incubated at 37°C in a 5% CO 2 humidified incubator for 20 h. Total protein lysates were generated using the passive lysis buffer provided with the Promega Dual Luciferase reporter assay system (E-1910).

    Article Title: Functional Genomic Characterization of mRNAs Associated with TcPUF6, a Pumilio-like Protein from Trypanosoma cruzi
    Article Snippet: .. T. cruzi epimastigotes (5 107) were transfected with 50 g of vector DNA by electroporation in 4-mm cuvettes, using the Bio-Rad GenePulser II electroporator, with two pulses of 450 V and 500 millifarads. ..

    Article Title: Small-Subunit rRNA Processome Proteins Are Translationally Regulated during Differentiation of Trypanosoma cruzi
    Article Snippet: .. Cells were transfected by electroporation in 4-mm cuvettes, using a Bio-Rad GenePulser II electroporator, with two pulses of 1,600 V and 25 F. After 24 h, 5 g/ml phleomycin was added for the selection of transfected parasites. ..

    Article Title: Epstein-Barr Virus Represses the FoxO1 Transcription Factor through Latent Membrane Protein 1 and Latent Membrane Protein 2A
    Article Snippet: .. For transient transfections, 1 107 DG75 cells from a suspension culture were transfected by electroporation using a Bio-Rad Genepulser II electroporator at 300 V and 950 F at room temperature in 500 l growth medium. .. Following electroporation, the cells were transferred to 3.5 ml of fresh medium per sample and incubated at 37°C in a 5% CO2 humidified incubator for 20 h. Total protein lysates were generated using the passive lysis buffer provided with the Promega Dual Luciferase reporter assay system (E-1910).

    Article Title: Functional Genomic Characterization of mRNAs Associated with TcPUF6, a Pumilio-like Protein from Trypanosoma cruzi
    Article Snippet: .. T. cruzi epimastigotes (5 × 10 7 ) were transfected with 50 μg of vector DNA by electroporation in 4-mm cuvettes, using the Bio-Rad GenePulser II electroporator, with two pulses of 450 V and 500 millifarads. ..

    Suspension:

    Article Title: Epstein-Barr Virus Represses the FoxO1 Transcription Factor through Latent Membrane Protein 1 and Latent Membrane Protein 2A
    Article Snippet: .. For transient transfections, 1 × 10 7 DG75 cells from a suspension culture were transfected by electroporation using a Bio-Rad Genepulser II electroporator at 300 V and 950 μF at room temperature in 500 μl growth medium. .. Following electroporation, the cells were transferred to 3.5 ml of fresh medium per sample and incubated at 37°C in a 5% CO 2 humidified incubator for 20 h. Total protein lysates were generated using the passive lysis buffer provided with the Promega Dual Luciferase reporter assay system (E-1910).

    Article Title: Epstein-Barr Virus Represses the FoxO1 Transcription Factor through Latent Membrane Protein 1 and Latent Membrane Protein 2A
    Article Snippet: .. For transient transfections, 1 107 DG75 cells from a suspension culture were transfected by electroporation using a Bio-Rad Genepulser II electroporator at 300 V and 950 F at room temperature in 500 l growth medium. .. Following electroporation, the cells were transferred to 3.5 ml of fresh medium per sample and incubated at 37°C in a 5% CO2 humidified incubator for 20 h. Total protein lysates were generated using the passive lysis buffer provided with the Promega Dual Luciferase reporter assay system (E-1910).

    Plasmid Preparation:

    Article Title: Functional Genomic Characterization of mRNAs Associated with TcPUF6, a Pumilio-like Protein from Trypanosoma cruzi
    Article Snippet: .. T. cruzi epimastigotes (5 107) were transfected with 50 g of vector DNA by electroporation in 4-mm cuvettes, using the Bio-Rad GenePulser II electroporator, with two pulses of 450 V and 500 millifarads. ..

    Article Title: Functional Genomic Characterization of mRNAs Associated with TcPUF6, a Pumilio-like Protein from Trypanosoma cruzi
    Article Snippet: .. T. cruzi epimastigotes (5 × 10 7 ) were transfected with 50 μg of vector DNA by electroporation in 4-mm cuvettes, using the Bio-Rad GenePulser II electroporator, with two pulses of 450 V and 500 millifarads. ..

    Selection:

    Article Title: Small-Subunit rRNA Processome Proteins Are Translationally Regulated during Differentiation of Trypanosoma cruzi
    Article Snippet: .. Cells were transfected by electroporation in 4-mm cuvettes, using a Bio-Rad GenePulser II electroporator, with two pulses of 1,600 V and 25 F. After 24 h, 5 g/ml phleomycin was added for the selection of transfected parasites. ..



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